Composite

Part:BBa_K1997021:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


P+R->sLuc-N->Zif268->sLuc-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1780
    Illegal BsaI.rc site found at 1513


Design Notes

We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced


Source

This part was synthesized by IDT

References